The overall objective of the Proteomics Shared Service (PSS) is to provide Arizona Cancer Center (AZCC) investigators with a dedicated facility and expertise in analyzing proteins for their identity, quantity and function via state-of-the art modern mass spectrometry and peripheral analytical instrumentation.
Over the past funding cycle, the PSS has perfomed proteomics analyses for AZCC members belonging to 5 different programs (units of service).
We offer a variety of proteomics services and tools to members of the AZCC.
- Gel Electrophoresis - used to separate proteins in a gel matrix
- 1D SDS-PAGE on 7 and 11 cm formats
- separation by MW
- coomassie, silver and Sypro Ruby images
- 1D SDS-PAGE on 7 and 11 cm formats
- 2D SDS-PAGE on 7 and 11 cm formats
- separation by pI and MW
- coomassie, silver and Sypro Ruby images
- Differential In-Gel Electrophoresis (DIGE) - expression analysis of complex protein samples
- Cy3, Cy5 and Cy2 labelling
- separation by pI and MW
- DeCyder expression analysis
- custom reports of expression differences
- statistics
- Protein MW determination
- ESI or MALDI ionization
- LC-MS/MS
- Digestion of protein in solution or in-gel
- nano HPLC introduction of peptides into mass spectrometer
- tandem mass spectrometry (MS/MS) peptide sequencing
- database searching
- Sequest, X!Tandem, MASCOT outputs
- SCAFFOLD visualization package
- secure on-line results on www.protbase.org
- LC-LC-MS/MS
- as LC-MS/MS but with dual nanoLC separation (Strong Cation Exchange and Reversed Phase chromatography)
- Albumin Depletion
- 12-most abundant protein depletion using FPLC
- Protein Purification
- combination of HPLC, gel filtrationa and FPLC (inquire with Core personnel)
- Phosphopeptide Enrichment
- Sample concentration and clean-up
For a full list of prices, please click here.
Prices are subject to change without notice and set by the University of Arizona Financial Services Office.