Flow Cytometry Shared Service

The Flow Cytometry Shared Service (FCSS) at the Arizona Cancer Center is highly utilized and supports research needs of Arizona Cancer Center members in several respects.  Techniques the service routinely performs are as follows: detection and analysis of cell surface markers, cancer chemotherapeutic agents, reporter dyes, intracellular proteins, cell cycle analysis/apoptosis studies, soluble cytokine detection and cell sorting.  A long-term goal of the service is to continue to provide state-of-the-art data collection, analysis and cell sorting capabilities to Arizona Cancer Center members.  To accomplish this goal, the service collects and analyzes data and performs cell sorting from 9:00 a.m. until 6:00 p.m.  It also works in consultation with investigators to develop and optimize new assays and techniques that become available for all users.  Because the FCSS processes many different samples from many different investigators, it can connect investigators interested in the same techniques.
  
Instrument use is nearly constant during working hours and sometimes after hours. Current user fees for the shared service are $42 per hour for analysis and $47 per hour for sorting. The flow cytometry specialist performs quality control every day to ensure that the FACScan and FACSAria instruments are calibrated properly.  The flow cytometry specialist plays a critical role in helping the facility run smoothly by solving scheduling problems, performing minor equipment maintenance, billing investigators, and helping users analyze data. 

The FCSS is a collaborative facility with the Cytometry Core Service of the Arizona Research Laboratories. For further information regarding FCSS instrumentation and links to specific flow cytometry resources, see Cytometry Core Facility .

 

Link to Resource Schedules

 

 

Service Director:  Katrina Trevor, Ph.D.
Facility Manager:  Barb Carolus

 

 

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